Infect Drug-Resistant Bacteria with Bacterial Viruses Found in Jerusalem Sewage

Hebrew U Scientists Infect Drug-Resistant Bacteria with Bacterial Viruses Found in Jerusalem Sewage

Every year, drug-resistant infections kill more than 50,000 people across Europe and the United States, and hundreds of thousands more around the world. According to theReview on Antimicrobial Resistance commissioned by the UK Prime Minister, failing to address the growing problem of drug-resistant infections could cause 10 million deaths a year and cost up to $100 trillion USD by 2050.

Now, researchers from the Hebrew University’sFaculty of Dental Medicine propose a way to turn the tables on harmful bacteria that infect humans by infecting them with tiny viruses called bacteriophages. In a strange twist, one such virus, cultivated from Jerusalem sewage, may help prevent infections following dental procedures.

Just a few decades ago, antibiotics were considered wonder drugs. Ironically, because they worked so well, they were used too often, leading to the rise of drug-resistant bacteria. These untreatable pathogens evolved mutations enabling them to resist the antibiotics that doctors prescribe to fight them.

One such pathogen is Enterococcus faecalis, a bacterium inhabiting the gastrointestinal tracts of humans. This life-threatening pathogen causes diseases ranging from endocarditis (a potentially fatal heart infection) to bacteremia (harmful bacteria in the bloodstream), as well urinary tract infection, meningitis, and post-treatment root canal infections.

The frustrating rate of infections reflects the limitations of current treatment options. Thus it is essential to develop additional ways to target highly virulent bacteria such as E. faecalis, especially when in biofilm form.

A promising alternative approach to traditional antibiotics is bacteriophage therapy. Bacteriophages, or “phages,” are viruses that infect bacteria. Phages play a key role in maintaining the natural balance in their predator-prey relationship with bacteria. They co-evolved with their bacterial hosts to be highly effective specific “professional bacterial killers.”

Now, a research team—led by Dr. Ronen Hazan from the Institute of Dental Sciences at the Hebrew University, and Dr. Nurit Beyth from the Hebrew University-Hadassah School Of Dental Medicine, together with their co-workers and students, Dr. Shunit Glaser, Leron Khalifa, Daniel Gelman and Yair Brosh—has identified a way to use phage therapy to target E. faecalis biofilms.

The researchers isolated an anti-E. faecalis phage from sewage effluents retrieved from a Jerusalem sewage treatment facility. The phage, named EFDG1, is capable of infecting the V583 strain of E. faecalis, which is resistant to vancomycin, the most effective anti-E. faecalis antibiotic.

Moreover, the researchers showed that EFDG1 was highly effective in root canal infection, both in vitro and ex vivo in tissue samples. These findings suggest that phage therapy using EFDG1 might be an effective way to prevent E. faecalis infection following root canal procedures.

According to Dr. Ronen Hazan, “The idea of using phages as anti-bacterial drugs is not new. Phage therapy was first proposed at the start of the 20th century, but then abandoned for various reasons, including the striking success of chemical antibiotics. Now we stand on the verge of a new era with the limitations of synthetic antibiotics and the emergence of antibiotic-resistant strains of bacteria. Thus it is the right time to look again into what Mother Nature offers in the battle against bacteria. As this research shows, bacteriophages may prove an effective tool in the development of much-needed new antimicrobial drugs.”

The research will appear in the April 2015 edition of Applied and Environmental Microbiology Journal as "Targeting Enterococcus faecalis biofilm using phage therapy"

The research was supported by a Hebrew University / Yissum startup grant.